autopurification hplcautolisp给排水系统图 多少钱

来源：蜘蛛抓取(WebSpider) 时间：2016-02-01 09:40 标签： water purification
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, November–December 1984, Pages
Purification of [125I]-vasoactive intestinal peptide by reverse-phase HPLC, , , , , , a Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190 USAb Veterans Administration Medical Center, Oklahoma City, OK 73190 USAVIP was labeled with sodium 125iodide, and 125I-VIP was purified by reverse-phase high performance liquid chromatography. Optimal separations of 125I-VIP and unlabeled VIP were obtained using two C18-Novapak columns in series and a gradient of acetonitrile in triethylamine phosphate for elution. The specific activity of the 125I-VIP was 1.99&0.21 Ci/μmole, approaching the maximum specific activity of monoiodinated VIP (2.26 Ci/μmole). Radioimmunoassay and radioreceptorassay for VIP were more sensitive (2.6-fold, and 2.5-fold, respectively) using 125I-VIP purified by HPLC compared to 125I-VIP obtained from an open-end cellulose column. These results demonstrate the advantage of preparing purified 125I-VIP by HPLC for the accurate assay of VIP and VIP-receptors in tissues and biological fluids.Keywords125I-VIP; High performance liquid chromatography; Radioimmunoassay; Radioreceptorassay
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No articles found.[Physico-chemical mechanisms of autopurification of sea-water] - Abstract - Europe PMC
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(PMID:5847841)
[):359-374]
Journal Article
(lang: fre)
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