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按字母分类 ：& 现货 瑞士ABB 5SHY35L4510 原装正品 IGCT系列
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现货 瑞士ABB 5SHY35L4510 原装正品 IGCT系列
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5shx14h4510
420A/4500V
产品型号 & & & &参数说明
5SHX19L60101单元，840A/5500V
5SHX06F60101单元，210A/5500V
5SHX14H45101单元，420A/4500V
5SHX10H60101单元，350A/5500V
5SHX26L45101单元，V
5SHX08F45101单元，250A/4500V
5SHY55L45001单元，V
5SHY35L45121单元，V
5SHY42L65001单元，V
5SHY35L45111单元，V
5SHY35L45101单元，V
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您当前访问的产品需要授权才能查看，请尝试用密码访问或索取密码。BioVector质粒载体菌种细胞基因保藏中心BioVector NTCC Inc.免费电话:400-800-2947010-Email:菌种:cDNA|基因库:细胞:蛋白/抗体:基因合成:订购:基因学院: >>&pIREShyg3质粒图谱序列抗性价格报价Biovector NTCC Inc.现货pIREShyg3质粒图谱序列抗性价格报价Biovector NTCC Inc.现货-->&竭诚为您服务！联系人：Dr.Xu, Biovector NTCC Inc.电话：免费电话订购:400-800-2947
北京实验室:010-邮件：手机：地址：北京已注册&载体基本信息ClontechpIREShyg3哺乳动物细胞表达载体CMV高多克隆位点，限制性内切酶5752 bpCMV-F: 5&#39;-CGCAAATGGGCGGTAGGCGTG-3&#39;pIRES-R:5&#39;-GCCCTAGATGCATGCTCG-3&#39;/AmpicillinHygromycinStably select with hygromycin631620稳表达 Stable组成型 Constitutive非病毒载体质粒图谱和多克隆位点信息载体简介DescriptionpIREShyg3 contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits the translation of two open reading frames from one messenger RNA (1–3). After selection with hygromycin B, nearly all surviving colonies will stably express the gene of interest, thus decreasing the need to screen large numbers of colonies to find functional clones. To select for cells that express high levels of the gene of interest, the selective pressure for antibiotic resistance was increased by shifting the hygromycin B phosphotransferase gene downstream to a less optimal position for translation as directed by the IRES sequence (3). By decreasing the level of expression of the antibiotic resistance marker, the selective pressure on the entire expression cassette is increased, resulting in selection for cells that express the entire transcript, including the gene of interest, at high levels. The expression cassette of pIREShyg3 contains the human cytomegalovirus (CMV) major immediate early promoter/enhancer followed by a multiple cloning site (MCS) that precedes stop codons in all three reading frames, a synthetic intron known to enhance the stability of the mRNA (4), the ECMV IRES followed by the hygromycin B phosphotransferase gene, and the polyadenylation signal from SV40. Ribosomes can enter the bicistronic mRNA at the 5&#39; end to translate the gene of interest and at the ECMV IRES to translate the antibiotic resistance marker. &UseWhen using the pIREShyg3 Vector, the antibiotic exerts selective pressure on the entire thus, a high dose of antibiotic will select for cells expressing a high level of the gene of interest. This selective pressure also ensures that the expression of the gene of interest will be stable over time in culture. Unless your expression experiments require a pure population of cells, you can use the pool of cells surviving selection instead of isolating and characterizing clonal cell lines. We recommend selecting mammalian cultures in 250–600 μg/ml Hygromycin B (Cat. No. 631309) depending on the cell line. Be sure to establish a kill curve for each cell line and each lot of Hygromycin B to determine optimal selection concentration.载体序列LOCUS & & & pIREShyg3 & &5752 bp & & DNA circular & &SYNDEFINITION &pIREShyg3ACCESSION & KEYWORDS & &SOURCE & & & &ORGANISM &
vectors.COMMENT & & This file is created by Vector NTI & & & & & &http://www.biovector.net/COMMENT & & ORIGDB|GenBankCOMMENT & & VNTAUTHORNAME|biovector.net|FEATURES & & & & & & Location/Qualifiers & & source & & & & &1..5752 & & & & & & & & & & /organism=&pIREShyg3& & & & & & & & & & & /mol_type=&other DNA& & & promoter & & & &236..852 & & & & & & & & & & /label=&CMV_immearly_promoter& & & misc_feature & &315..602 & & & & & & & & & & /label=&CAG_enhancer& & & misc_feature & &769..789 & & & & & & & & & & /label=&CMV_fwd_primer& & & promoter & & & &863..881 & & & & & & & & & & /label=&T7_promoter& & & misc_feature & & & & & & & & & & & & /label=&synth_int& & & misc_feature & & & & & & & & & & & & /label=&IRES& & & CDS & & & & & &
& & & & & & & & & & /label=&ORF frame 1& & & & & & & & & & & /translation=&MLYMCLVEVKKTSRPPEPRGRGFPLKNTMISLPQPVPKMDRSGK & & & & & & & & & & PELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDR & & & & & & & & & & YVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAM & & & & & & & & & & DAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQA & & & & & & & & & & LDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFW & & & & & & & & & & RPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRC & & & & & & & & & & DAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*& & & CDS & & & & & & complement() & & & & & & & & & & /label=&ORF frame 2& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & gene & & & & & & & & & & & & & & & & /label=&hygroB& & & & & & & & & & & /gene=&hygroB& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & terminator & & & & & & & & & & & & & /label=&SV40_PA_terminator& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & misc_feature & & & & & & & & & & & & /label=&EBV_rev_primer& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & misc_feature & & & & & & & & & & & & /label=&EBV_rev_primer& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & promoter & & & &complement() & & & & & & & & & & /label=&M13_reverse_primer& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & misc_feature & &complement() & & & & & & & & & & /label=&M13_pUC_rev_primer& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & promoter & & & &complement() & & & & & & & & & & /label=&lac_promoter& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & rep_origin & & &complement() & & & & & & & & & & /label=&pBR322_origin& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & gene & & & & & &complement() & & & & & & & & & & /label=&Ampicillin& & & & & & & & & & & /gene=&Ampicillin& & & & & & & & & & & /translation=&MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA & & & & & & & & & & AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA & & & & & & & & & & YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT & & & & & & & & & & AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES & & & & & & & & & & TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP & & & & & & & & & & WFGGPRRFFNLD*& & & CDS & & & & & & complement() & & & & & & & & & & /label=&ORF frame 2& & & & & & & & & & & /translation=&MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY & & & & & & & & & & IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE & & & & & & & & & & YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL & & & & & & & & & & DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL & & & & & & & & & & LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA & & & & & & & & & & EIGASLIKHW*& & & promoter & & & &complement() & & & & & & & & & & /label=&AmpR_promoter& & & & & & & & & & & /translation=&MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY & & & & & & & & & & IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE & & & & & & & & & & YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL & & & & & & & & & & DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL & & & & & & & & & & LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA & & & & & & & & & & EIGASLIKHW*&ORIGIN & &1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG & 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG &121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC &181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT &241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA &301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC &361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC &421 ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT &481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT &541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA &601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG &661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC &721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG &781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA &841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC &901 GAGCTCGGAT CTGTACAGGC CTTAAGCCGG CGCGCTAGCG CTTCGAAGGC GCCCGGGATC &961 CGATATCGTA CGTTAACATA GATAACTGAT CCAGTGTGCT GGAATTAATT CGCTGTCTGC 1021 GAGGGCCAGC TGTTGGGGTG AGTACTCCCT CTCAAAAGCG GGCATGACTT CTGCGCTAAG 1081 ATTGTCAGTT TCCAAAAACG AGGAGGATTT GATATTCACC TGGCCCGCGG TGATGCCTTT 1141 GAGGGTGGCC GCGTCCATCT GGTCAGAAAA GACAATCTTT TTGTTGTCAA GCTTGAGGTG 1201 TGGCAGGCTT GAGATCTGGC CATACACTTG AGTGACAATG ACATCCACTT TGCCTTTCTC 1261 TCCACAGGTG TCCACTCCCA GGTCCAACTG CAGGTCGAGC ATGCATCTAG GGCGGCCGCA 1321 CTAGAGGAAT TCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG 1381 GAATAAGGCC GGTGTGTGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC 1441 AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC 1501 CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA 1561 GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT 1621 GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA 1681 CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA 1741 AGCGTAGTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGAATCTGA 1801 TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG 1861 CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAG CTTGCCACAA 1921 CCCGTACCAA AGATGGATAG ATCCGGAAAG CCTGAACTCA CCGCGACGTC TGTCGAGAAG 1981 TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA GGGCGAAGAA 2041 TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT AAATAGCTGC 2101 GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC CGCGCTCCCG 2161 ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG CATCTCCCGC 2221 CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC TGTTCTGCAG 2281 CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC GAGCGGGTTC 2341 GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT CATATGCGCG 2401 ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT CAGTGCGTCC 2461 GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA AGTCCGGCAC 2521 CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG CATAACAGCG 2581 GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC CAACATCTTC 2641 TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA GCGGAGGCAT 2701 CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG TCTTGACCAA 2761 CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA GGGTCGATGC 2821 GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA CACAAATCGC CCGCAGAAGC 2881 GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG ATAGTGGAAA CCGACGCCCC 2941 AGCACTCGTC CGAGGGCAAA GGAATAGAGT AGATGCCGAC CGAACAAGAG CTGATTTCGA 3001 GAACGCCTCA GCCAGCAACT CGCGCGAGCC TAGCAAGGCA AATGCGAGAG AACGGCCTTA 3061 CGCTTGGTGG CACAGTTCTC GTCCACAGTT CGCTAAGCTC GCTCGGCTGG GTCGCGGGAG 3121 GGCCGGTCGC AGTGATTCAG GCCCTTCTGG ATTGTGTTGG TCCCCAGGGC ACGATTGTCA 3181 TGCCCACGCA CTCGGGTGAT CTGACTGATC CCGCAGATTG GAGATCGCCG CCCGTGCCTG 3241 CCGATTGGGT GCAGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT 3301 TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA 3361 ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC 3421 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC 3481 ATCAATGTAT CTTAACGCTC GAGTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 3541 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA 3601 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 3661 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 3721 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 3781 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 3841 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 3901 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 3961 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 4021 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 4081 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 4141 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG 4201 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 4261 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 4321 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 4381 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 4441 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 4501 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 4561 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 4621 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 4681 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 4741 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 4801 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 4861 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 4921 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 4981 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 5041 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 5101 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 5161 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 5221 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 5281 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 5341 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 5401 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 5461 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 5521 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 5581 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 5641 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 5701 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TC用户内容询问日期您正在向&&发送关于产品&&的询问*联系人电话E-mail留言点击“立即发送”后，我们将在1个工作日内与您取得联系。&
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