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来源：蜘蛛抓取(WebSpider) 时间：2017-07-19 06:12 标签： 苹果id登录服务器出错
The level of APOBEC3G (hA3G)-related G-to-A mutations does not correlate with viral load in HIV type 1-infected individuals.
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):1285-90. doi: 10.1089/aid..The level of APOBEC3G (hA3G)-related G-to-A mutations does not correlate with viral load in HIV type 1-infected individuals.1, , , , , .1Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA.AbstractThe APOBEC family of mammalian cytidine deaminases, such as APOBEC3G (hA3G), has been demonstrated to function as a host viral restriction factor against HIV-1. hA3G has been shown to cause extensive G-to-A mutations in the HIV-1 genome, which may play a role in viral restriction. To investigate the role of G-to-A mutations in HIV-1 pathogenesis, we isolated, amplified, and sequenced HIV-1 sequences (vif, gag, and env) from 29 therapy-naive HIV-1-infected individuals. The levels of G-to-A mutations correlated with the expression levels of hA3G in the vif (rho = 0.438, p = 0.041) and the env regions (rho = 0.392, p = 0.038), but not in the gag region (rho = 0.131, p = 0.582). There is no correlation between viral load and the level of G-to-A mutations in the vif (rho = 0.144, p = 0.522), env (rho = 0.168, p = 0.391), or gag regions (rho = -0.254, p = 0.279). Taken together, these findings suggest that the hA3G-induced G-to-A mutations may not be the mechanism by which hA3G restricts or controls viral replication. Thus, hA3G might be restricting viral growth in infected individuals through a mechanism that is independent of the cytidine deaminase activities of hA3G.PMID:
[Indexed for MEDLINE] Histogram depicting the percentage of GG-to-AG, GA-to-AA, and GY-to-AY mutations in the vif, env, and gag sequences. Mutation occurring at GG, GA, and GY dinucleotide contexts are indicated by black, gray, and white, respectively.AIDS Res Hum Retroviruses. ):.Correlations of the levels of G-to-A mutations [in vif (a), env (b), and gag (c)] and the amount of hA3G mRNA. Correlation coefficients (rho) and p values were calculated using Spearman' p values &0.05 were considered significant.AIDS Res Hum Retroviruses. ):.Publication typeMeSH termsSubstancesSecondary source IDGrant supportFull Text SourcesOther Literature SourcesMedicalMolecular Biology Databases
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