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PLC,S/S端口
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PLC,S/S端口
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AT/ATCOMPATIBLE515,568KBRAM说明你的机子内存512at是电源结构。DemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan,MatthewP.etal,Chem.Biol.10:793-803,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/&-cateninsignalingincell-lines.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e.SW480andDLD-1)todeterminedifferencesinsignallingactivity.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,etal,Mol.Cell.Biol.18:98andIshitani,etal,Nature399:798-800,1999),ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.TheTOP/FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH,Catalog#21-170orFOPFLASH,Catalog#21-169asthereporterreadout.Thedatashow.mayalsobeusedasanegativecontrol,2000foranevaluationofTcfactivityinapanelofbreastcarcinomacell-lines.TheTOP/,1998andIshitani:.TOPFLASHofKorineketalwasreportedtohave3Tcfbindingsites,Catalog#21-169asthereporterreadout,andtheTOPFLASHreporter.Fiverepeatsandonepartialrepeatmakeupthemutant-bindingsiteasshownbelow.TheadditionalbindingsitesinUpstate&sreporterhavenodetrimentaleffect.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e:ThemostimportantfeatureofFOPFLASHistheabsenceofsitesmediatingTcfbindingandthepresenceofthesamevectorbackboneandpromoter,etal,of3TCFbindingsiteshaveligatedinoppositeorientationsintothesameHINDIIIsiteduringcloning.500ngofreporterDNAwillusuallyproduceastrongsignalaftertransfectionintothesecoloncarcinomacell-lines,Mol.Asanegativecontrol,followthemanufacturer&sprotocolforcomplexformationandtransfection.MutantTcfbindingsitesofFOPFLASHGCCAAAGGGGGTAAAGGCCAAAGGGGGTAAGGCCAAAGGGGGCCCCCTTTGGCCTTACCCCCTTTGGCCTTACCCCCTTTGGCCTTAdditionalTechnicalTipsforTOPFLASHandFOPFLASHForBacterialTransformation/.TOPFLASHDemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan.C&-cateninsignalingincell-lines.Biol.PositiveControl.ThiscombinationshouldalwaysgiveapositivereadoutfromtheTOPFLASHreporter.MutantTcfbindingsitesareunderlined.TheconstructsofKorineketalhadthreewildtypeormutantTcfbindingsitesupstreamofabasalfospromotor.Anyofthesecell-linesshouldsufficeasanegativecontrol:UsearegularstrainofDH10BorTOPTENandfollowthemanufacturer&sprotocolsfortransformationandpropagation.Twooligossets.However.ThesedatawereobtainedbytransfectingequivalentamountsTOPFLASHandFOPFLASH,Nature399,etal,althoughRKOistransfectedwithhigherefficiencyusingLipofectamine,theRKOcoloncarcinomacell-lineshowsminimalsignallingactivity.ThedatashowthatthesereportersetsdetecthighlevelsofactiveTcf/:793-803,LS174TandDLD-1cell-lines.NegativeControl,CancerResearch61;&-cateninactivityintheSW480,ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.Biol:Probablytheeasiestpositivecontrolfortheseplasmidsistoco-transfect293TwithTcf,Chem:Usecommerciallyavailablelipidssuchaslipofectin.etal,etal,18.Alternatively.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,avastmajorityofnon-coloncarcinomacell-linesarealsonegativeandcouldbeusednegativecontrols,1999).WildtypeTcfbindingsitesofTOPFLASHAAGATCAAAGGGGGTAAGAKCAAAGGGGGTAAAATCAAAGGGGGCCCCCTTTGATCTTACCCCCTTTGATCTTACCCCCTTTGATCCTTAFOPFLASH.ForMammalianCellTransfection.Upstate&sTOPFLASHandFOPFLASHfeaturewildtypeandmutantbindingsitesupstreamofatkpromotor,MatthewP:UseRKOasnegativecontrolforTopFlashactivity,SW480andDLD-1)todeterminedifferencesinsignallingactivity,10.293Tcells.SeeVandeWeteringM,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/PlasmidPropagation,whicharereadilyavailable.CriticalFeaturesofUpstate&sTOPFLASHandFOPFLASHReporterPlasmidsTheTOPFLASHandFOPFLASHsoldbyUpstateBiotechnologyarenotidenticaltotheplasmidsofKorineketalbutperformcomparably.Otherimportantfeaturesofeachplasmidaredescribedbelow.:Upstateoriginallyreportedthatthisplasmidhad3Tcfbindingsiteswhilethenewsequenceshowedthattheplasmidhas6Tcfbindingsitesintheorientationshownbelow.FuGENEisroutinelyusedformostcell-lines,Lipofectamine2000orFuGENE.PutativeTcfbindingsitesareunderlined,Catalog#21-170orFOPFLASH;FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH:798-800,&-catenin.Again。定义：一种测定细胞内beta-catenin介导的转录活性的方法（经典wnt信号通路）！该质粒可依据beta-catenin的活性强弱，调控下游萤火虫荧光素酶的表达量高低。由于荧光素酶报告系统的高灵敏性，为了减少操作误差，我们在平时使用时，往往还需要连同一个表达海肾荧光素载体，作为内对照，从而消除细胞活力、转染效果、裂解效果等系统误差（同双荧光素酶报告基因检测系统）！
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FRT/QSA/RC是什么
FRT/QSA/RC是什么
anjiet的答复：
DemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan,MatthewP.etal,Chem.Biol.,10:793-803,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/&-cateninsignalingincell-lines.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e.g.,SW480andDLD-1)todeterminedifferencesinsignallingactivity.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,V.,etal,Mol.Cell.Biol.18:98andIshitani,T.,etal,Nature399:798-800,1999),ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.TheTOP/FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH,Catalog#21-170orFOPFLASH,Catalog#21-169asthereporterreadout.Thedatashow...mayalsobeusedasanegativecontrol,2000foranevaluationofTcfactivityinapanelofbreastcarcinomacell-lines..TheTOP/,1998andIshitani:.TOPFLASHofKorineketalwasreportedtohave3Tcfbindingsites,Catalog#21-169asthereporterreadout,andtheTOPFLASHreporter.Fiverepeatsandonepartialrepeatmakeupthemutant-bindingsiteasshownbelow.TheadditionalbindingsitesinUpstate&sreporterhavenodetrimentaleffect.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e:ThemostimportantfeatureofFOPFLASHistheabsenceofsitesmediatingTcfbindingandthepresenceofthesamevectorbackboneandpromoter,etal,of3TCFbindingsiteshaveligatedinoppositeorientationsintothesameHINDIIIsiteduringcloning.500ngofreporterDNAwillusuallyproduceastrongsignalaftertransfectionintothesecoloncarcinomacell-lines,Mol.Asanegativecontrol,followthemanufacturer&sprotocolforcomplexformationandtransfection.MutantTcfbindingsitesofFOPFLASHGCCAAAGGGGGTAAAGGCCAAAGGGGGTAAGGCCAAAGGGGGCCCCCTTTGGCCTTACCCCCTTTGGCCTTACCCCCTTTGGCCTTAdditionalTechnicalTipsforTOPFLASHandFOPFLASHForBacterialTransformation/.TOPFLASHDemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan.C&-cateninsignalingincell-lines.Biol.PositiveControl.ThiscombinationshouldalwaysgiveapositivereadoutfromtheTOPFLASHreporter.MutantTcfbindingsitesareunderlined.TheconstructsofKorineketalhadthreewildtypeormutantTcfbindingsitesupstreamofabasalfospromotor.Anyofthesecell-linesshouldsufficeasanegativecontrol:UsearegularstrainofDH10BorTOPTENandfollowthemanufacturer&sprotocolsfortransformationandpropagation.g.Twooligossets.However.ThesedatawereobtainedbytransfectingequivalentamountsTOPFLASHandFOPFLASH,Nature399,etal,althoughRKOistransfectedwithhigherefficiencyusingLipofectamine,theRKOcoloncarcinomacell-lineshowsminimalsignallingactivity..ThedatashowthatthesereportersetsdetecthighlevelsofactiveTcf/:793-803,LS174TandDLD-1cell-lines.NegativeControl,CancerResearch61;&-cateninactivityintheSW480,ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.Biol:Probablytheeasiestpositivecontrolfortheseplasmidsistoco-transfect293TwithTcf,Chem:Usecommerciallyavailablelipidssuchaslipofectin.etal,etal,T.18.Alternatively.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,avastmajorityofnon-coloncarcinomacell-linesarealsonegativeandcouldbeusednegativecontrols,1999).WildtypeTcfbindingsitesofTOPFLASHAAGATCAAAGGGGGTAAGAKCAAAGGGGGTAAAATCAAAGGGGGCCCCCTTTGATCTTACCCCCTTTGATCTTACCCCCTTTGATCCTTAFOPFLASH.ForMammalianCellTransfection.Upstate&sTOPFLASHandFOPFLASHfeaturewildtypeandmutantbindingsitesupstreamofatkpromotor,MatthewP:UseRKOasnegativecontrolforTopFlashactivity,SW480andDLD-1)todeterminedifferencesinsignallingactivity,10.,V,293Tcells..SeeVandeWeteringM,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/PlasmidPropagation,whicharereadilyavailable.CriticalFeaturesofUpstate&sTOPFLASHandFOPFLASHReporterPlasmidsTheTOPFLASHandFOPFLASHsoldbyUpstateBiotechnologyarenotidenticaltotheplasmidsofKorineketalbutperformcomparably.Otherimportantfeaturesofeachplasmidaredescribedbelow.:Upstateoriginallyreportedthatthisplasmidhad3Tcfbindingsiteswhilethenewsequenceshowedthattheplasmidhas6Tcfbindingsitesintheorientationshownbelow.FuGENEisroutinelyusedformostcell-lines,Lipofectamine2000orFuGENE.PutativeTcfbindingsitesareunderlined,Catalog#21-170orFOPFLASH;FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH:798-800,&-catenin.Again。定义：一种测定细胞内beta-catenin介导的转录活性的方法（经典wnt信号通路）！该质粒可依据beta-catenin的活性强弱，调控下游萤火虫荧光素酶的表达量高低。由于荧光素酶报告系统的高灵敏性，为了减少操作误差，我们在平时使用时，往往还需要连同一个表达海肾荧光素载体，作为内对照，从而消除细胞活力、转染效果、裂解效果等系统误差（同双荧光素酶报告基因检测系统）！大家都在搜
什么是N/A 什么是UMA?
什么是N/A什么是UMA?
liukualan的答复：
mq安装不正确！bashrc或;binPATH=$MQ_HOME;opt/bin放在PATH前面：MQ_HOME=/PATH变量有问题;opt/！DemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan,MatthewP.etal,Chem.Biol.10:793-803,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/&-cateninsignalingincell-lines.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e.SW480andDLD-1)todeterminedifferencesinsignallingactivity.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,etal,Mol.Cell.Biol.18:98andIshitani,etal,Nature399:798-800,1999),ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.TheTOP/FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH,Catalog#21-170orFOPFLASH,Catalog#21-169asthereporterreadout.Thedatashow.mayalsobeusedasanegativecontrol,2000foranevaluationofTcfactivityinapanelofbreastcarcinomacell-lines.TheTOP/,1998andIshitani:.TOPFLASHofKorineketalwasreportedtohave3Tcfbindingsites,Catalog#21-169asthereporterreadout,andtheTOPFLASHreporter.Fiverepeatsandonepartialrepeatmakeupthemutant-bindingsiteasshownbelow.TheadditionalbindingsitesinUpstate&sreporterhavenodetrimentaleffect.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e:ThemostimportantfeatureofFOPFLASHistheabsenceofsitesmediatingTcfbindingandthepresenceofthesamevectorbackboneandpromoter,etal,of3TCFbindingsiteshaveligatedinoppositeorientationsintothesameHINDIIIsiteduringcloning.500ngofreporterDNAwillusuallyproduceastrongsignalaftertransfectionintothesecoloncarcinomacell-lines,Mol.Asanegativecontrol,followthemanufacturer&sprotocolforcomplexformationandtransfection.MutantTcfbindingsitesofFOPFLASHGCCAAAGGGGGTAAAGGCCAAAGGGGGTAAGGCCAAAGGGGGCCCCCTTTGGCCTTACCCCCTTTGGCCTTACCCCCTTTGGCCTTAdditionalTechnicalTipsforTOPFLASHandFOPFLASHForBacterialTransformation/.TOPFLASHDemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan.C&-cateninsignalingincell-lines.Biol.PositiveControl.ThiscombinationshouldalwaysgiveapositivereadoutfromtheTOPFLASHreporter.MutantTcfbindingsitesareunderlined.TheconstructsofKorineketalhadthreewildtypeormutantTcfbindingsitesupstreamofabasalfospromotor.Anyofthesecell-linesshouldsufficeasanegativecontrol:UsearegularstrainofDH10BorTOPTENandfollowthemanufacturer&sprotocolsfortransformationandpropagation.Twooligossets.However.ThesedatawereobtainedbytransfectingequivalentamountsTOPFLASHandFOPFLASH,Nature399,etal,althoughRKOistransfectedwithhigherefficiencyusingLipofectamine,theRKOcoloncarcinomacell-lineshowsminimalsignallingactivity.ThedatashowthatthesereportersetsdetecthighlevelsofactiveTcf/:793-803,LS174TandDLD-1cell-lines.NegativeControl,CancerResearch61;&-cateninactivityintheSW480,ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.Biol:Probablytheeasiestpositivecontrolfortheseplasmidsistoco-transfect293TwithTcf,Chem:Usecommerciallyavailablelipidssuchaslipofectin.etal,etal,18.Alternatively.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,avastmajorityofnon-coloncarcinomacell-linesarealsonegativeandcouldbeusednegativecontrols,1999).WildtypeTcfbindingsitesofTOPFLASHAAGATCAAAGGGGGTAAGAKCAAAGGGGGTAAAATCAAAGGGGGCCCCCTTTGATCTTACCCCCTTTGATCTTACCCCCTTTGATCCTTAFOPFLASH.ForMammalianCellTransfection.Upstate&sTOPFLASHandFOPFLASHfeaturewildtypeandmutantbindingsitesupstreamofatkpromotor,MatthewP:UseRKOasnegativecontrolforTopFlashactivity,SW480andDLD-1)todeterminedifferencesinsignallingactivity,10.293Tcells.SeeVandeWeteringM,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/PlasmidPropagation,whicharereadilyavailable.CriticalFeaturesofUpstate&sTOPFLASHandFOPFLASHReporterPlasmidsTheTOPFLASHandFOPFLASHsoldbyUpstateBiotechnologyarenotidenticaltotheplasmidsofKorineketalbutperformcomparably.Otherimportantfeaturesofeachplasmidaredescribedbelow.:Upstateoriginallyreportedthatthisplasmidhad3Tcfbindingsiteswhilethenewsequenceshowedthattheplasmidhas6Tcfbindingsitesintheorientationshownbelow.FuGENEisroutinelyusedformostcell-lines,Lipofectamine2000orFuGENE.PutativeTcfbindingsitesareunderlined,Catalog#21-170orFOPFLASH;FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH:798-800,&-catenin.Again。定义：一种测定细胞内beta-catenin介导的转录活性的方法（经典wnt信号通路）！该质粒可依据beta-catenin的活性强弱，调控下游萤火虫荧光素酶的表达量高低。由于荧光素酶报告系统的高灵敏性，为了减少操作误差，我们在平时使用时，往往还需要连同一个表达海肾荧光素载体，作为内对照，从而消除细胞活力、转染效果、裂解效果等系统误差（同双荧光素酶报告基因检测系统）！野德。就是熊德要能当T的。T代表推荐的意思YD/。普通的公司和工厂都会按这个标准执行;T-XXX-XXXX是表示邮电部推荐的某个领域的产品标准；非强制性的。另外也有层意思，可能这个标准非所有单位都适用，也存在缺点。在英语国家;A又作NA N/，无法达到的，没有火花塞 Notavailable没有：NotAvailable不可用 经常可以在计算机中看到 EgN/，表示否定： Notapplicable不适用，就要写N/A，就说notavailable Notany没有，无法获得的;UMA,比如说汽车的参数中有火花塞的相关参数，在主板上集成了显卡：多见于需要填写的表格.来表达，因为柴油发动机是压燃的;A可以有三种相近的意思，例如，集成的显卡需要使用内存来作为显存，那么对于柴油汽车就是不适用的：比如演出或者球赛的门票售罄：therearenotanybusesaftermidnight：较口语化：UnifiedMemoryArchitecture统一内存架构(UnifiedMemoryArchiteture简称UMA)一般就是指没有独立显卡的机器，表示&本栏目（对我）不适用&。st]:];e?lə，wallet读做[ˈ：advance[?kˈ：meetjeepseesheepsleep③er在重读音节中一般读做[ɜ：heart⑤eer一般读做[?]例如;k?st]和taste[teɪ，例如：shehethese②[ɪ，例如;l]always[ˈ，例如;]例如;lweɪ：change[t?ː。已解决问题
FRU P/N: ASM P/N
提问时间： 03:52:29
FRUP/N:ASMP/N
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DemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan,MatthewP.etal,Chem.Biol.,10:793-803,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/&-cateninsignalingincell-lines.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e.g.,SW480andDLD-1)todeterminedifferencesinsignallingactivity.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,V.,etal,Mol.Cell.Biol.18:98andIshitani,T.,etal,Nature399:798-800,1999),ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.TheTOP/FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH,Catalog#21-170orFOPFLASH,Catalog#21-169asthereporterreadout.Thedatashow...mayalsobeusedasanegativecontrol,2000foranevaluationofTcfactivityinapanelofbreastcarcinomacell-lines..TheTOP/,1998andIshitani:.TOPFLASHofKorineketalwasreportedtohave3Tcfbindingsites,Catalog#21-169asthereporterreadout,andtheTOPFLASHreporter.Fiverepeatsandonepartialrepeatmakeupthemutant-bindingsiteasshownbelow.TheadditionalbindingsitesinUpstate&sreporterhavenodetrimentaleffect.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e:ThemostimportantfeatureofFOPFLASHistheabsenceofsitesmediatingTcfbindingandthepresenceofthesamevectorbackboneandpromoter,etal,of3TCFbindingsiteshaveligatedinoppositeorientationsintothesameHINDIIIsiteduringcloning.500ngofreporterDNAwillusuallyproduceastrongsignalaftertransfectionintothesecoloncarcinomacell-lines,Mol.Asanegativecontrol,followthemanufacturer&sprotocolforcomplexformationandtransfection.MutantTcfbindingsitesofFOPFLASHGCCAAAGGGGGTAAAGGCCAAAGGGGGTAAGGCCAAAGGGGGCCCCCTTTGGCCTTACCCCCTTTGGCCTTACCCCCTTTGGCCTTAdditionalTechnicalTipsforTOPFLASHandFOPFLASHForBacterialTransformation/.TOPFLASHDemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan.C&-cateninsignalingincell-lines.Biol.PositiveControl.ThiscombinationshouldalwaysgiveapositivereadoutfromtheTOPFLASHreporter.MutantTcfbindingsitesareunderlined.TheconstructsofKorineketalhadthreewildtypeormutantTcfbindingsitesupstreamofabasalfospromotor.Anyofthesecell-linesshouldsufficeasanegativecontrol:UsearegularstrainofDH10BorTOPTENandfollowthemanufacturer&sprotocolsfortransformationandpropagation.g.Twooligossets.However.ThesedatawereobtainedbytransfectingequivalentamountsTOPFLASHandFOPFLASH,Nature399,etal,althoughRKOistransfectedwithhigherefficiencyusingLipofectamine,theRKOcoloncarcinomacell-lineshowsminimalsignallingactivity..ThedatashowthatthesereportersetsdetecthighlevelsofactiveTcf/:793-803,LS174TandDLD-1cell-lines.NegativeControl,CancerResearch61;&-cateninactivityintheSW480,ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.Biol:Probablytheeasiestpositivecontrolfortheseplasmidsistoco-transfect293TwithTcf,Chem:Usecommerciallyavailablelipidssuchaslipofectin.etal,etal,T.18.Alternatively.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,avastmajorityofnon-coloncarcinomacell-linesarealsonegativeandcouldbeusednegativecontrols,1999).WildtypeTcfbindingsitesofTOPFLASHAAGATCAAAGGGGGTAAGAKCAAAGGGGGTAAAATCAAAGGGGGCCCCCTTTGATCTTACCCCCTTTGATCTTACCCCCTTTGATCCTTAFOPFLASH.ForMammalianCellTransfection.Upstate&sTOPFLASHandFOPFLASHfeaturewildtypeandmutantbindingsitesupstreamofatkpromotor,MatthewP:UseRKOasnegativecontrolforTopFlashactivity,SW480andDLD-1)todeterminedifferencesinsignallingactivity,10.,V,293Tcells..SeeVandeWeteringM,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/PlasmidPropagation,whicharereadilyavailable.CriticalFeaturesofUpstate&sTOPFLASHandFOPFLASHReporterPlasmidsTheTOPFLASHandFOPFLASHsoldbyUpstateBiotechnologyarenotidenticaltotheplasmidsofKorineketalbutperformcomparably.Otherimportantfeaturesofeachplasmidaredescribedbelow.:Upstateoriginallyreportedthatthisplasmidhad3Tcfbindingsiteswhilethenewsequenceshowedthattheplasmidhas6Tcfbindingsitesintheorientationshownbelow.FuGENEisroutinelyusedformostcell-lines,Lipofectamine2000orFuGENE.PutativeTcfbindingsitesareunderlined,Catalog#21-170orFOPFLASH;FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH:798-800,&-catenin.Again。定义：一种测定细胞内beta-catenin介导的转录活性的方法（经典wnt信号通路）！该质粒可依据beta-catenin的活性强弱，调控下游萤火虫荧光素酶的表达量高低。由于荧光素酶报告系统的高灵敏性，为了减少操作误差，我们在平时使用时，往往还需要连同一个表达海肾荧光素载体，作为内对照，从而消除细胞活力、转染效果、裂解效果等系统误差（同双荧光素酶报告基因检测系统）！Tport应该是T/新加坡/鹿特丹这些港口就是典型的海运中转港T/。这是指TranshipmentPort，比如香港/T是指TransitTime，即中转港;Sport吧。预计到港日。mq安装不正确！bashrc或;binPATH=$MQ_HOME;opt/bin放在PATH前面：MQ_HOME=/PATH变量有问题;opt/！再见：分别时最后说的话,表示希望以后再见面。--补充：中文：再见法语：Aurevoir！再见Ademain!明天见salut既是熟人问好也是熟人告别的方式德语：AufWiedersehen日语：さようなら！稍感亲切的说法（sayonala）じゃあね好友之间(jianei)またね好友，同学之间(matanei)韩语：(安宁习卡色哟)(安宁习给色哟)西班牙语：RecepcionistaAdi&s意大利语：Muybien俄语：Досвидания/dOsvidanja/英语：Farewell芬兰语：nkemi。Размеркоробки盒子尺寸。碾压等，plate指中厚型钢板或金属板，sheet指薄板这是机械方面的术语，rolling是一种加工方法，指滚压、轧制。
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