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Type C virus particles in phytohaemagglutinin-stimulated spleen cell cultures from a leucotic calf.
Wittmann, W.; Solisch, P.1972Archiv fur Experimentelle Veterinarmedizin 26(Heft 1): 111-114Type C virus particles in phytohaemagglutinin-stimulated spleen cell cultures from a leucotic calf.
Spleen cell cultures from a cow with tumorous leucosis, stimulated with phytohaemagglutinin, produced typical type C particles found in similarly treated lymphocyte cultures described by other authors.
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Enzymatic characterization of the chondrocytic alkaline phosphatase ec 3.1.3.1 isolated from bovine fetal epiphyseal cartilage
Fortuna, R.; Anderson, H.C.; Carty, R.; Sajdera, S.W.1979Biochimica et Biophysica Acta 570(2): 291-302Enzymatic characterization of the chondrocytic alkaline phosphatase ec 3.1.3.1 isolated from bovine fetal epiphyseal cartilage
Purified chondrocytic alkaline phosphatase (orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1) from bovine fetal epiphyseal cartilage hydrolyzes a variety of phosphate esters, ATP and PPi. Optimal activities for p-nitrophenyl phosphate, ATP and PPi are found at pH 10.5, 10.0 and 8.5, respectively. The latter 2 substrates exhibit substrate inhibition at high concentrations. p-Nitrophenyl phosphate demonstrates decreasing pH optima with decreasing substrate concentration. Heat inactivation studies indicate that phosphorolytic and pyrophosphorolytic cleavage occur at the same site on the enzyme. Mg2+ (0.1-10.0 mM) and Mn2+ (0.01 mM) show a small stimulation of p-nitrophenyl phosphate-splitting activity at pH 10.5. Levamisole, Pi, CN-, Zn2+ and L-phenylalanine are all reversible inhibitors of the phosphomonoesterase activity. Pi is a competitive inhibitor with a Ki [inhibition constant] of 10.0 mM. Levamisole and Zn2+ are potent non-competitive inhibitors with Ki of 0.05 and 0.04 mM, respectively. The chondrocytic alkaline phosphatase is inhibited irreversibly by Be2+, EDTA, EGTA [ethyleneglycol-bis [.beta.-aminoethyl ether] N,N,N',N'-tetraacetic acid], ethane-1-hydroxydiphosphonate, dichloromethane diphosphonate, L-cysteine, phenylmethylsulfonyl fluoride, N-ethylmaleimide and iodoacetamide. NaCl, KCl and Na2SO4 at 0.05-1.0 M inhibit the enzyme. At pH 8.5, the cleavage of PPi (pyrophosphate phosphohydrolase, EC 3.6.1.1) by the condrocytic enzyme is slightly enhanced by low levels of Mg2+ and depressed by concentrations higher than 1 mM. Ca2+ shows only inhibition. Similar effects of Mg2+ and Ca2+ on the associated ATPase (ATP phosphohydrolase, (EC 3.6.1.3) activity were observed. Arrhenius studies using p-nitrophenyl phosphate and AMP as substrates accounted for the 10-fold difference in V [velocity] in terms of small differences in the enthalpies and entropies of activation which are 700 cal/mol and 2.3 cal/degree per mol, respectively.
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Response of scoliotic spines to segmental spinal instrumentation
Osebold W.R.; Yamamoto S.; Merkel C.; Hurley J.H.1990Orthopaedic Transactions 14(3): 807Response of scoliotic spines to segmental spinal instrumentation
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Iron and magnesium distribution between orthopyroxene-calcic pyroxene and olivine-orthopyroxene in chondritic meteoritics
VanSchmus W.R.; Koffman D.M.1968Special Paper - Geological Society of America Pages 228-229Iron and magnesium distribution between orthopyroxene-calcic pyroxene and olivine-orthopyroxene in chondritic meteoritics
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Mites of the genus Dermatophagiodes in dust from the houses of asthmatic and other allergic patients in North America: development of a radioimmunoassay for allergen produced by D. farinae and/or D. pteronyssinus
Platts Mills, T.A.E.; Heymann, P.W.; Chapman, M.D.; Smith, T.F.; Wilkins, S.R.1985International Archives of Allergy and Applied Immunology 77(1-2): 163-165Mites of the genus Dermatophagiodes in dust from the houses of asthmatic and other allergic patients in North America: development of a radioimmunoassay for allergen produced by D. farinae and/or D. pteronyssinus
The development of a radioimmunoassay for detecting antigens of D. farinae and D. pteronyssinus in house dust is discussed in the light of recent research on the subject. Preliminary results from houses in Virginia and Georgia, USA, are given.
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